Representative appressorium stage cDNA library ofMagnaporthe grisea
نویسندگان
چکیده
منابع مشابه
cDNA Subtractive Cloning of Genes Expressed during Early Stage of Appressorium Formation by Magnaporthe grisea.
The conidial germ tube of the fungus Magnaporthe grisea differentiates an infection-specific structure, an appressorium, for penetration into the host plant. Formation of the appressorium is also observed on synthetic solid substrata such as polycarbonate. We found that a plant lectin, concanavalin A, specifically suppressed the appressorium formation without affecting the germling adhesion if ...
متن کاملConstruction of a representative cDNA library from prostatic intraepithelial neoplasia.
We report the construction of a plasmid-based cDNA library made from microdissected cells derived from prostatic intraepithelial neoplasia. Total RNA was extracted and converted to blunt-ended, double-stranded cDNA by oligo(dT)-mediated reverse transcription followed by linker addition. A linker-specific primer with UDG-compatible ends was used to amplify the cDNA and the resulting PCR product ...
متن کاملMstu1, an APSES transcription factor, is required for appressorium-mediated infection in Magnaporthe grisea.
The APSES protein family includes important transcriptional regulators of morphological processes in ascomycetes. We identified a deletion mutant of the APSES protein Mstu1 in Magnaporthe grisea that showed reduced conidiation and mycelial growth. Mstu1 formed a number of appressoria comparable to the wild type, although appressorium formation was delayed. In M. grisea, rapid transfer of conidi...
متن کاملThe molecular biology of appressorium turgor generation by the rice blast fungus Magnaporthe grisea.
The rice blast fungus Magnaporthe grisea develops specialized infection structures known as appressoria, which develop enormous turgor pressure to bring about plant infection. Turgor is generated by accumulation of compatible solutes, including glycerol, which is synthesized in large quantities in the appressorium. Glycogen, trehalose and lipids represent the most abundant storage products in M...
متن کاملMagnaporthe grisea cutinase2 mediates appressorium differentiation and host penetration and is required for full virulence.
The rice blast fungus Magnaporthe grisea infects its host by forming a specialized infection structure, the appressorium, on the plant leaf. The enormous turgor pressure generated within the appressorium drives the emerging penetration peg forcefully through the plant cuticle. Hitherto, the involvement of cutinase(s) in this process has remained unproven. We identified a specific M. grisea cuti...
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ژورنال
عنوان ژورنال: Journal of Zhejiang University SCIENCE
سال: 2005
ISSN: 1009-3095
DOI: 10.1631/jzus.2005.b0132